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Study of de novo purine synthesis in model systems of Caenorhabditis elegans and HeLa cell lines
Součková, Olga ; Zikánová, Marie (advisor) ; Šebesta, Ivan (referee) ; Kučera, Lukáš (referee)
Purines are involved in many biologic processes and are required to maintain stable energy charge, for metabolic regulation, as cofactors in enzymatic reactions and as building blocks in DNA and RNA. Necessary role in purine synthesis is played by de novo purine synthesis (DNPS) that is highly active in developing cells. DNPS is complex pathway involving enzymes that assemble into multienzyme complex, purinosome, which facilitates flux of purine intermediates through sequence of ten enzymatically catalyzed reactions. Properly functioning enzymes and purinosome ensure the fast flux of unstable and potentially cytotoxic intermediates resulting in final product IMP, the branch point for AMP and GMP synthesis. The mutations in DNPS genes lead to inherited rare disorders that are accompanied by elevated concentrations of enzyme substrates in body fluids and cells. Lack of a suitable model system to study pathophysiology of DNPS raised the necessity to develop a system mimicking DNPS disorders. HeLa cells with malfunctioning DNPS were characterized on the level of genes, transcripts, proteins, metabolites, and for the presence of the purinosome. Homozygous or compound heterozygous mutations led to the absence of proteins, decreased enzymatic activities, accumulation of enzyme substrates, downregulation...
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